Pentraxins are a family of proteins emerging from genes that are conserved in their carboxy-terminal halves a pentraxin domain and are prototypical acute phase proteins with acquired novel amino-terminal domains.
Pentraxins, include C reactive protein (CRP) and Serum Amyloid P component (SAP), which serve as indicators of inflammatory reactions as a result of the exposure of liver cells to cytokines, mainly interleukin-6 (IL-6).
PTX3 or TSG14 or long pentraxin (human mature chain 364aa, chromosome 3q25; ~43kD) is structurally related but different form classic pentraxins.
PTX3 was clones as an IL-1 inducible gene in endothelial cells, and as TNF-inducible gene in fibroblasts called TSG14.
The C-terminal half of PTX3 aligns with CRP and SAP, but the N-terminal half is unique.
PTX3 is induced by LPS, IL-1 and TNF-alpha, but not IL-6.
During an acute phase response induced by LPS, PTX3 is expressed in many organs (heart, skeletal muscle).
PTX3 expression may be indicative of its role in cardiovascular and inflammatory pathology.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/ml (ECL)ELISA: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human PTX3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human PTX3.
