84-1175-88　Prolactin (PRL) 100ul　P9009-15A

特徴

• Prolactin (PRL) is a polypeptide hormone synthesized by the lactotropic cells of the anterior pituitary glad.
• Structurally, it is similar to two other polypeptide hormones namely, growth hormone and placental lactogen.
• PRL is a polypeptide hormone containing 199aa, while growth hormone and placental lactogen each have 191aa.
• There is about 100 ug of prolactin in the human pituitary gland, which is very small amount when compared to Growth hormone, which is present in 8-10mg.
• Prolactin target organ is the breast (mammary gland).
• Its main physiological action is not only to initiate but also to sustain lactation.
• The hypothalamus secretes dopamine, which has a direct effect of inhibition on the secretion of PRL.
• If dopamine is not available or absent the secretion of PRL is autonomous.
• PRL increases during sleep and pregnancy.
• In women giving birth and they do not breast feed then PRL decreases to its normal level in a short time.
• During breast feed, PRL remains elevated for about 3 months.
• Also during pregnancy the size of the pituitary gland increases which is due to the proliferation of lactotropic cells.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-5ug/ml (ECL)ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.