Control of appetite involves a number of gastrointestinal hormones that belong to the ghrelin-motilin ligand family that either stimulate food intake and gastric emptying or inhibit these responses.
Recently, a new member of the ghrelin-motilin family was identified by using bioinformatic predictions about enzyme cleavage of the prepropeptide of ghrelin.
The newly identified 23aa, ghrelin-associated peptide was named obestatin.
Although both peptides originate from the same precursor prepropeptide, they have opposing physiological roles.
Obestatin exerts anorexigenic effects by decreasing food intake, gastric emptying, jejunal motility, and weight gain by binding to GPR39 orphan receptor, while ghrelin exerts opposing orexigenic effects by binding to growth hormone secretagogue receptor.
Obestatin: Human, mouse, rat 23aa each - 2.6kD; mouse Chromosome: 6E3.
Mainly expressed in the gastrointestinal tract with higher levels in the stomach, medium levels in the duodenum, jejunum, ileum and colon.
Low expression in the testis and brain.
Not detected in the salivary gland, pancreas, liver and lung.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000Western Blot: 1-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes mouse Obestatin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 23aa of mouse Obestatin (Accession #Q9EQX0), N-terminal, extracellular (KLH).
