84-1175-77　Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Neural Enolase, Enolase 2, ENO2) 100ul　N2175-02R

特徴

• The glycolytic enzyme enolase (2-phospho-Dglycerate hydrolyase) exists as several dimeric isoenzymes (aa, ab, ag, bb and gg) composed of three distinct subunits: a, b, and g (alpha, beta, and gamma).
• Three isoenzymes are found in human brain: aa, ag, and gg.
• The ag and gg-enoloase isoenzymes are also known as neuron- specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells.
• Lung cancer is one of the most spread cancer forms with incidences about 50-100 per 100,000 population.
• Approximately 20% of the lung cancer is small cell lung cancer.
• NSE has been shown to be a valuable tumor marker of neuroendocrine origin, particulary in small cell lung cancer and in neuroblastoma.
• Patients with small cell lung cancer show various proportions of ag and gg isoenzymes.
• The determination of NSE should detect ag and gg isoforms with the same sensitivity.
• The antibodies for this particular assay are specific for the g-subunit without cross reactivity with a or b subunits.
• NSE is reported to be useful diagnostic marker for lung cancer, neuroblastoma, melnoma, seminoma and in injury of central nervous system.
• In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:100,000Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.