84-1175-76　NCKX3 (Sodium/Potassium/Calcium Exchanger 3, a(+)/K(+)/Ca(2+)-exchange Protein 3, Solute Carrier Family 24 Member 3, SLC24A3) 100ug　N0559-06A

特徴

• Ca2+ plays a critical role in intracellular signaling.
• Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and the Na+/Ca2+ exchangers (NCX), in the plasma membrane.
• NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane.
• In most cells, 3 Na+ are exchanged for 1 Ca2+.
• Several genes code for the 3 NCX (NCX1, NCX2, and NCX3), and three in the NCKX family (NCKX1, NCKX2, NCKX3).
• NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus.
• The N-terminal signal peptide is cleaved off from the mature exchanger protein.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-1ug/ml.
• Control peptide can be used to coat ELISA plate at 1ug/mlOptimal dilutions to be determined by the researcher.
• Control Peptide:N0559-06B: NCKX3, Control Peptide
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.