E.coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA.
The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity.
The post-replicative adenine:8-oxo-7,8-dihydroguanine GO) mismatch is crucial for G:C to T:A transversion.
This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO.
A candidate gene coding for the human counterpart of MutY has been cloned as hMYH (535aa).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 (Chemiluminescence)ELISA: 1:10,000-1:50,000 (using 50-100ng control antigen/well)
Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human mutY. No significant homology with E. coli mutY.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 18aa from human mutY (KLH).
