Human Leptin is an 167aa, ~16kD, adipocyte derived, secreted protein.
Mouse Leptin was cloned, expressed in E.
Coli and purified.
The purity of Leptin is greater than 95% as determined by SDS-PAGE.
The identity of protein has been confirmed by N-Terminal sequence analyses.
Endotoxin level in the final preparation is less than 1pg/ug of murine Leptin.
Mouse leptin is about 85% homologous with human Leptin.
Purified Mouse Leptin has been shown to be biologically active in reducing body weight, food consumption, and plasma glucose levels in ob/ob and NZO mice.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-50,000 (10-100ng of protein/well)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes mouse leptin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to mouse leptin.
