84-1175-66　Liver-expressed Antimicrobial Peptide 2 (LEAP-2) 100ul　L1600-20B

特徴

• Human genome contains numerous genes whose protein products are unknown in terms of structure, interaction partner, expression, and function.
• From human blood ultrafiltrate, a novel gene-encoded, cysteine-rich, and cationic peptide termed as liver- expressed antimicrobial peptide 2 (LEAP-2) together with several circulating forms of LEAP-2 differing in their amino-terminal length were identified, all containing a core structure with two disulfide bonds formed by cysteine residues in relative 1-3 and 2-4 positions.
• The largest native LEAP-2 form of 40aa residues is generated from the precursor at a putative cleavage site for a furin-like endoprotease.
• In contrast to smaller LEAP-2 variants, this peptide exhibited dose-dependent antimicrobial activity against selected microbial model organisms.
• LEAP2: rat= 75aa; mouse= 76aa; human= 77aa; Human chromosome: 5q31.1.
• Protein homology between mose, rat and human LEAP2 is 99%.
• LEAP2 is expressed in liver, blood, urine and kidney.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000 (50-100ng control peptide/well)Western Blot: 1-10ug/ml (chemiluminescence)Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.