84-1175-61　IRS-2 (Insulin Receptor Substrate 2) 100ul　I8700-15B

特徴

• Insulin receptor substrate (IRS) proteins play a pivotal role in signal transduction by the receptors for insulin, insulin-like growth factor 1 (IGF-1), and a growing number of cytokines and integrins.
• Four IRS proteins (IRS-1, IRS-2, IRS-3 and IRS-4) have beenidentified and characterized.
• Recently, two more signaling proteins, IRS5/DOK4 and IRS6/DOK5, have been identified as potentially new members of the IRS family.
• IRSs are tyrosine-phosphorylated following insulin or IGF-1 stimulation and form signaling complexes at the receptor with Src homology 2 domain-containing proteins, including the p85 regulatory subunit of phosphatidylinositol 3-kinase, Grb2, Nck, and Shc.
• IRS family of proteins share a number of structural and functional characteristics, including an N-terminal pleckstrin homology (PH) and a phosphotyrosine binding domain (PTB), followed by a large C-terminal region containing multiple sites of tyrosine phosphorylation, which serve as docking sites for Src homology 2 domain-containing signaling proteins.
• Despite the overall similarity, the IRS proteins differin their subcellular distribution and tissue/developmental expression pattern.
• Each IRS is phosphorylated on a set of specific tyrosine residues, leading to its binding to the Src homology 2 domains of distinct signaling proteinsand therefore, activation of specific tyrosine residues, leading to its binding to the Src homology 2 domains of distinct signaling proteins and therefore, activation of specific signaling cascades.
• Disruption of each individual IRS gene causes distinct phenotypes in mice, further indicating that the four IRS proteins play different roles in the regulation of the pleiotropic effects of insulin, IGF-1, and other growth factors.
• Applications:Suitable for use in Immunoprecipitation and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunoprecipitation: 2-10ug or ul using 500ug lysate (control cells 3T3/A31 RIPA lysate)
• Western Blot: 1:500-1:2000 or 1-5 ug/ml (chemiluminescence).
• Expected band ~170-185kD
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.