84-1175-57　IGFBP-1 (Insulin-like Growth Factor-binding Protein 1, IBP-1, IGF-binding Protein 1, Placental Protein 12, PP12, IGFBP1, IBP1) 100ul　I7661-16B10

特徴

• Unlike insulin, both IGF-I and IGF-II circulate in plasma tightly bound to specific binding proteins.
• Two major forms of IGFbinding proteins have been identified in human plasma, a low molecular weight form and a high molecular weight form.
• The low molecular weight IGF-binding protein (IGFBP1) is synthesized in liver, secretory endometrium, and decidua.
• It binds both IGF I and IGF II with high affinity.
• Insulin-like growth factor binding protein-1 IGFBP-1 ~30-kD; also known as IBP1 or placental protein 12, or IGF-BP25; chromosome 7p14-p12)) is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high-affinity IGF binding proteins (IGFBP1-6) and at least four low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP).
• The IGFBP members are cysteinerich proteins with conserved cysteine residues, clustered in the amino-terminal and the carboxy-terminal regions of the molecule.
• Contained within IGFBP-1 and –2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF-independent action.
• IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment.
• IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF-independent manner.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:5,000 (ECL technique)ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.