Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or carboxy-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices.
Therefore, tags enable a selective identification and purification of the protein of interest.
Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions.
This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns.
Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/mlImmunohistochemistry: 1:100-1:500Immunofluorescence: 1:100-1:500ELISA: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.
