84-1175-54　Hexokinase 4 (Glucokinase, Hexokinase Type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, GCK) 100ul　H2040-51M

特徴

• Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P.
• Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate.
• At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized.
• Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins.
• The N-terminus, containing the mitochondrial target sequence, and the C- terminal has high sequence homology among various isoforms.
• The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus.
• HXK4 (mouse 465aa, isoforms 1) isoforms are produced by use of alternative promoters.
• The use of alternative promoters apparently enables the type IV hexokinase gene to be regulated by insulin in the liver and glucose in the beta cell.
• This may constitute an important feedback loop for maintaining glucose homeostasis.
• HXK4 isoform 2 (missing 122-138aa) and HXK4-3 (isoforms 3) containes a different N- terminus from 1-15aa (MLDDRARMEATKKEK- >MAMDTTRCGAQLLTL).
• Alternative names for HXK4.
• EC 2.7.1.1, Hexokinase type IV, HK IV, HK4, Glucokinase (Gck).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.