84-1175-50　Hexokinase 3 (HXK3, HK3, Hexokinase Type III, HK III) 100ug　H2035-36

特徴

• Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P.
• Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate.
• At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized.
• Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins.
• The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms.
• The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus.
• HXK3 (chromosome 5) has a more limited distribution that includes white cells and shows a genetically determined polymorphism.
• It is also expressed in lung and liver.
• It is 50% identical to HXK1.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-1ug/ml; Coat ELISA plates at 1ug/ml with control peptide
• Western Blot: 1-10ug/ml using chemiluminescence
• Optimal dilutions to be determined by the researcher.
• Control Peptide:H2035-36A: Hexokinase 3, Control Peptide (HXK3, HK3, Hexokinase Type III, HK III)
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.