84-1175-49　Hexokinase 2 (HXK2, HK2, Hexokinase Type II, HK II, Muscle Form Hexokinase) 100ul　H2035-31

特徴

• Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P.
• Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate.
• At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized.
• Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins.
• The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms.
• The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus.
• HXKII is located on chromosome 2.
• HXKII is the predominant isozyme that is expressed in young red cell, the skeletal muscle, adipose tissue, and reticulocytes.
• It is regulated by insulin.
• Defects in HXKII are a cause of monogenic autosomal dominant non-insulin-dependent diabetes mellitus type II (MODY-II or NIDDM).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.