84-1175-42　GPR39 (G-Protein Coupled Receptor 39, GPCR39) 100ul　G8600-43K

特徴

• Control of appetite involves a number of gastrointestinal hormones that belong to the ghrelin-motilin ligand family that either stimulate food intake and gastric emptying or inhibit these responses.
• These hormones have been the focus of intense research in hopes of developing drug therapy for gastrointestinal and eating disorders.
• GPR39 is a G protein-coupled receptor (GPCR).
• It is sequentially distantly related to the growth hormone secretagogue receptor (ghrelin) and neurotensin receptors.
• Its endogenous ligand was unknown until 2005, when an amidated 23aa peptide, obestatin, was found to bind to it specifically.
• Interestingly, these two peptides appear to have opposite effects.
• While ghrelin stimulates appetite by acting on its receptor in the arcuate nucleus, obestatin suppresses appetite.
• GPR39 mouse: 456aa; human: 453aa -51kD; Chromosome: 2q21-q22.
• Expressed in many tissues and brain regions.
• GPR39 is alternatively spliced.
• Full length GPR39-1a (456aa in mouse with 7 TM domains) and truncated form (GPR39-1b; 315aa with 5 TM domains).
• Quantitative RT-PCR analysis demonstrated that GPR39-1a is expressed selectively throughout the gastrointestinal tract, including the liver and pancreas as well as in the kidney and adipose tissue, whereas the truncated GPR39-1b form has a more broad expression pattern, including the central nervous system but with highest expression in the stomach and small intestine.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000Western Blot: 1-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.