84-1175-35　VGLUT3 (Vesicular Glutamate Transporter 3, Solute Carrier Family 17 Member 8, SLC17A8) 100ug　G4007-02

特徴

• Vesicular glutamate transporter (VGLUT) is responsible for the active transport of L-glutamate in synaptic vesicles and thus plays an essential role in the glutamatergic chemical transmission in the central nervous system.
• VGLUT comprises three isoforms, VGLUT1, 2, and 3, and is a potential marker for the glutamatergic phenotype.
• Recent studies indicated that VGLUT is also expressed in non-neuronal cells, and localized with various organelles such as synaptic-like microvesicles in the pineal gland, and hormone- containing secretory granules in endocrine cells.
• L-Glutamate is stored in these organelles, secreted upon various forms of stimulation, and then acts as a paracrine-like modulator.
• Thus, VGLUTs highlight a novel framework of glutamatergic signaling and reveal its diverse modes of action.
• VGLUT3 expression is restricted to the brain, where it is predominantly located in synaptic vesicles.
• VGLUT3 shows strong sequence homology (~73%) to Differentiation-associated Na- dependent inorganic phosphate cotransporter.
• Mouse and rat respectively show 90 and 92% identity with the human VGLUT3 protein sequence.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000; 50-100ng of G4007-02A control peptide/wellOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.