Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol.
Wt.
40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and C-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Glut isoform differ in their tissue expression, substrate specificity and kinetic characteristics.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000ELISA: 1:10,000-1:50,000 (using 1ug/ml control peptide to coat ELISA plates)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human GLUT7.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 12aa from human GLUT7, C-terminal, Cytoplasmic domain (KLH).
