84-1175-31　GLUT 4 (Glucose Transporter 4, Glucose Transporter Type 4 Insulin-responsive, GLUT4, GLUT-4, Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 4, SLC2A4) 100ug　G3900-50J

特徴

• Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
• Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol.
• Wt.
• 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD).
• Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
• Both N- and C-termini are predicted to be cytoplasmic.
• Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
• Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
• Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
• Glut-2 provides glucose to the liver and pancreatic cells.
• Glut-3 is the main transporter in neurons, whereas Glut-4 (mouse/human 509aa) is primarily expressed in muscle and adipose tissue and regulated by insulin.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-1ug/ml, Control peptide (Cat #G3900-50K) can be used to coat ELISA plates at 1ug/mlImmunohistochemistry: 2-20ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.