84-1175-30　GLUT1 (Glucose Transporter 1, Solute Carrier Family 2, Facilitated Glucose Transporter Member 1, Glucose Transporter Type 1, Erythrocyte/Brain, GLUT-1, HepG2 Glucose Transporter, SLC2A1) 100ul　G3900-05B

特徴

• Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
• Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol.
• Wt.
• 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD).
• Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
• Both N and c-termini are predicted to be cytoplasmic.
• Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue- specific targeting.
• Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
• Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-1:50,000.
• Control peptide can be used to coat plates at 1ug/mlWestern Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
• Control Peptide:G3900-05L: GLUT 1 (Glucose Transporter 1) Control Peptide
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.