84-1175-25　Glucokinase Regulatory Protein (GCKR, GKRP, Glucokinase Regulator) 100ul　G2023-37

特徴

• Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P.
• Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate.
• At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized.
• Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins.
• The N- terminus, containing the mitochondrial target sequence, and the C- terminal has high sequence homology among various isoforms.
• The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus.
• Glucokinase (GCK) in the liver and pancreatic beta cells is subject to inhibition by a regulatory protein, GCKR or GKRP.
• The inhibitory effect of GCKR depends on the presence of fructose-6-phosphate and is antagonized by fructose-1-phosphate.
• Mutations in GCKR might be diabetogenic if they resulted in the synthesis of proteins with increased inhibitory activity, perhaps reflecting increased sensitivity to fructose-6-phosphate or reduced susceptibility to antagonism by fructose-1-phosphate.
• The GCKR/GKRP gene encodes a protein of 625aa.
• Given the role of glucokinase in the causation of maturity-onset diabetes of the young (MODY) type II.
• GCKR had been considered a candidate gene for a form of MODY.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (ECL)ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.