GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
GABA exerts its effects through ionotropic ligand- gated GABAA, GABAC and GABAB receptors (GABABRs).
A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric) protein complexes.
Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role.
GABA A (GAA) receptor proteins (450-627aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM (transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain.
The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits, which may confer subunit specific functionality.
GAA genes are distributed as clusters throughout the human genome (chromosomes 4, 5, 15, and X; delta subunit on chromosome 1).
GAA in the brain are the targets of many clinically important drugs.
Human (chromosome Xq28), rat GAA3 are 493aa protein.
GAA3 may be candidate gene for X-linked manic depressive illness, which has been mapped to the Xq28 region close to color blindness and G6PD.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000; 50-100ng control peptide/well
Western Blot: 1-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes rat GABA-A Receptor Alpha 3 Subunit. Species Crossreactivity: human. Species sequence homology: mouse.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to 13aa of rat GAA3, N-terminal, extracellular domain (KLH).
