Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells.
This process is termed vasculogenesis.
A further remodeling of the primitive vascular system forms the mature cardiovascular system.
This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature).
Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF).
VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR-1 (flt-1 or fms-like tyrosine kinase 1), VEGFR-2 (flk-1 or fetal liver kinase-1/KDR or Kinase insert domain containing receptor), and VEGFR-3/Flt-4.
Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo.
FLK-1 (Fetal-Liver Kinase 1; human homolog is KDR, Kinase insert domain containing receptor or VEGF-R2; mouse 1345aa; rat 1343aa, and human 1356), a putative receptor protein tyrosine kinase.
FLK-1 belongs to the family of type-1 membrane protein.
Human FLK-1 protein: 1-19 signal peptide, 20-1356 mature protein (20-764 extracellular domain, 765-789 TM, and 790-1356 cytoplasmic domain).FLK-1 Receptor for VEGF or VEGFC.
Has a tyrosine-protein kinase activity.
The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
It is expressed in endothelial cells of developing embryo.
It is also expressed at high levels in adult heart, lung, kidney, brain and skeletal muscle, but is also expressed at lower levels in most other adult tissues.
Applications:Suitable for use in Western Blot, ELISA, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1200-1:500 (Chemiluminescence)ELISA: 1:10,000-1:100,000; 50-100ng of control peptide/wellImmunohistochemistry: 2-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes mouse FLK1. Species Crossreactivity: rat and human.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 20aa of mouse FLK1, C-terminal, Cytoplasmic domain (KLH).
