Fatty acids are important for general cellular metabolism.
FABPs fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD) and proteins that has widespread tissue distribution.
FABPs are quite abundant (3-5% of total cellular protein).
At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues.
FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine.
Several different isoform of FABP have been identified and generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain etc; protein designated as H-FABP indicates that it is heart type).
However, expression of these isoform is not exclusive and more than one isoform can be found in a given cell or tissue.
Three main types of FABPs that were initially discovered in the heart (FABP-H), liver (FABP-L), and intestine (FABP-I) are not exclusive these tissues and show considerable differences at the aa level (~30% identity).
Other FABPs recently detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with other FABPs.
FABPs are also known as mammary derived growth inhibitor (MDGI), adipocyte lipid binding protein (ALBP), Myelin protein P2 homolog, P2 adipocyte protein, 422 protein (P15).
Human FABP-H or adipocyte is 132aa protein (chromosome 2p11).
Rat and mouse adipocyte-FABPs are 133aa single polypeptide chains.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-10,000Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human FABP. Cross-reactivity: 100% homology with 132aa human adipocyte lipid binding protein (hALBP) and mouse/rat H-FABPs, 91% homology with human muscle FABP, porcine and bovine H-FABPs.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 12aa of human FABP (heart/adipocyte) (KLH).
