D1 dopamine receptor-interacting protein calcyon D1IP) is a type II single transmembrane protein.
D1IP belongs to neron-specific gene (NSG) family of proteins.
It is required for maximal stimulated calcium release after stimulation of purinergic or muscarinic but not betaadrenergic receptors.
The encoded protein interacts with D1 dopamine receptor (drd1) and may interact with other dopamine receptor subtypes and/or GPCRs.
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
D1IP (rat: 226aa, mouse: 226aa; human 217aa, 23.4kD, human chromosome 10q26.3) D1IP2 is expressed in the pyramidal cells of prefrontal cortex, in hypothalamus and in caudate nucleus.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml (chemiluminescence)ELISA: 1:100,000, using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human D1IP.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 17aa of human D1IP (UniProt #Q9NYX4) (KLH).
