Asthma is a chronic lung disease characterized by airway hyper-responsiveness (AHR) to allergens, airway edema, and increased mucus secretion.
Increased levels of circulating IgE and IgG1 antibody and a propensity to allergic responses, atopy, are associated with the development of asthma.
Animal models of AHR, where control of the timing of exposure to the initiating antigen, the use of a defined allergen trigger, and genetic manipulation are likely to enhance the understanding of AHR.
Dinitrophenol (DNP) has long been employed as a model immunogen as a single antigen to reduce the complexity of modeling AHR.
One of the primary aspects of the immune response is the interaction of antigens with lymphocytes to induce the formation of antibodies, that in turn makes the antigen harmless.
Much of our current understanding of the antibody response to antigens has been derived by using the antibody-hapten model.
One known model uses the dinitrophenyl (DNP) group.
Immunization of many mammalian species with DNP-protein conjugates results in production of antibodies specific for DNP and the aa side chains to which it is attached.
DNP conjugates (DNP-KLH or DNP-BSA) are useful for the production of antibodies specific for DNP and hemocyanin or BSA.
DNP immunization produced a significant variation in the amount and antibody class (IgGs, IgA, IgE, IgM) among strains, and under various experimental conditions.
DNP preparations (purity and supplier), routes (intramuscular, intravenous, polymerized, etc), frequency of exposure (single injections, multiple etc) may induce a defined class of antibody and its level may vary as well.
DNP-induced antibody production has been used to assess the immune status of normal and immune compromised animals.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:10,000Western Blot: 1:200-1:2000 using ECL
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：mouse
Grade：Ascites
Purity：Ascites
Form：Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS, pH 7.4.
Specificity：Recognizes DNP-modified proteins. May also react with KLH protein.
