In the eukaryotic cell, DNA primase initiates DNA replication by the synthesis of small ribonucleotides called primers.
The eukaryotic primase is composed of two subunits, p49 (human 420aa; chromosome 12q13) and p58 (human 509aa, chromosome 6p12-p11.1), which purify as a complex tightly bound to DNA polymerase alpha.
Primase initiates synthesis with a triphosphate purine moiety at the 5'-end.
After synthesis of 7-10 ribonucleotides, the primer-template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit.
The p49 subunit of DNA primase contains the catalytic active site.
Residue 104-111 are most critical for primer synthesis.
Alanine substitution in residues Glu, Asp, and these residues may form part of a conserved carboxylic triad also observed in the active sites of DNA polymerases and reverse transcriptases.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa cells or or other tumor cells.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes mouse DNA Primase. Species crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant corresponding to mouse DNA Primase, p49.
