RNA editing is an important mechanism for regulating genetic plasticity through the generation of alternative protein products from a single structural gene.
There are two types of substitutional RNA exist in mammals, namely A-to-I and C-to-U RNA editing.
The best-characterized example of C-to-U RNA editing involves the nuclear transcript encoding intestinal apolipoprotein B (apo B)).
Apo B RNA editing changes a CAA to a UAA stop codon, generating a truncated protein, apoB48.
The functional complex includes a minimal core composed of apobec-1 and ACF, that function as an adaptor protein by binding both the deaminase and the RNA substrate.
The RNA binding proteins also include CUGBP2 which along with Apobec-1 binds to the consensus binding sequence UUUN (A/U) U, present in c-myc, VEGF and Cyclooxygenase-2 (COX2).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml (ECL)ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human CUGBP2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 18aa of human CUGBP2 (KLH).
