Pentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions.
C-reactive protein (CRP or PTX1; mature chain 206aa; chromosome 1q21-23) nonglycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates.
Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the pneumococcus.
It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation; in the case of CRP the rise may be 1000-fold or more.
CRP is composed of 5 identical, 21,500-molecular weight subunits.
It is detectable on the surface of about 4% of normal peripheral blood lymphocytes.
Acute phase reactant CRP is produced in the liver; those cells produce CRP detectable on lymphocytes.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:5000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
