Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2.
Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin.
C3 is an acute phase reactant.
Synthesis of C3, a glycoprotein, is induced during acute inflammation.
The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages.
A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1663aa.
This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds.
Pro-C3 contains a signal peptide of 22aa residues, the beta chain (645 residues) and the alpha chain (992 residues).
The 2 chains are joined by 4 arginine residues that are not present in the mature protein.
Human C3 has 79% identity to mouse C3 at the nucleotide level and 77% at the amino acid level.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:5000 (ECL)ELISA: 1:10,000-50,000 (50-100ng control antigen/well)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
