84-1175-08　Complement C3 (C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, C3, CPAMD1) 100ug　C7850-12H

特徴

• Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2.
• Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin.
• C3 is an acute phase reactant.
• Synthesis of C3, a glycoprotein, is induced during acute inflammation.
• The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages.
• A single chain precursor (pro-C3) of approximately 200 kD is found intracellularly; the cDNA shows that it comprises 1,663aa.
• This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds.
• Pro-C3 contains a signal peptide of 22aa residues, the beta chain (645 residues) and the alpha chain (992 residues).
• The 2 chains are joined by 4 arginine residues that are not present in the mature protein.
• Human C3 has 79% identity to mouse C3 at the nucleotide level and 77% at the aa level.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000 (ECL)ELISA: 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.