Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2.
Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin.
C3 is an acute phase reactant.
Synthesis of C3, a glycoprotein, is induced during acute inflammation.
The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages.
A single chain precursor (pro-C3) of approximately 200 kD is found intracellularly; the cDNA shows that it comprises 1,663aa.
This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds.
Pro-C3 contains a signal peptide of 22aa residues, the beta chain (645 residues) and the alpha chain (992 residues).
The 2 chains are joined by 4 arginine residues that are not present in the mature protein.
Human C3 has 79% identity to mouse C3 at the nucleotide level and 77% at the aa level.
Human C3 concentration in normal human serum is ~1.25mg/ml.
Classical and alternative activation pathways of complement converge at C3 step.
Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces.
Both C3 convertases cleave the C3 a-chain at peptide bond 77 resulting in production of C3a (Molecular Weight 9083) and C3b fragments (Molecular Weight 80,000).
Released C3a peptide is one of the three complement anaphylatoxins.
The nascent C3b fragment can form a covalent ester bond with target surface.
This covalent attachment of C3b to target acceptors is required for continuation of complement activation.
C3 nephritic factor, an IgG antibody against complement components, is demonstrable in some cases of partial lipodystrophy.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-50,000 50-100ng control antigen/wellWestern Blot: 1:1000-1:5000 (chemiluminescence technique)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA.
