84-1175-01　CISY (Citrate Synthase, Mitochondrial, CS) 100ul　C5700-04B

特徴

• The enzyme citrate synthase (E.C.
• 2.3.3.1 [previously 4.1.3.7]) is a pace-maker enzyme, as it controls the first committed step of the Krebs cycle, also called the citric acid cycle.
• Citrate synthase is localized within cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial.
• It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix.
• Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria.
• Citrate synthase catalyzes the condensation reaction of the two-carbon acetate residue from acetyl coenzyme A and a molecule of four-carbon oxaloacetate to form the sixcarbon citrate.
• Oxaloacetate will be regenerated after the completion of one round of the Krebs Cycle.
• Human CS (chromosome 12p11-qter, 466aa precursor, mature chain 28-466aa) encodes a 466aa protein with 95% homology to its pig homolog.
• CS enzyme, a homodimer, is located at the Mitochondrion matrix.
• Citrate synthase is found in nearly all cells capable of oxidative metabolism.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-5000ELISA: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.