coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues.
Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification.
In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag- epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene.
Proteins are expressed as fusion proteins.
Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification.
Therefore, fusion-tags serve as universal tags much like secondary antibodies.
Many tags have their own characteristics.
Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP.
GST-fusion proteins can bind to glutathione-Sepharose.
Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step.
Purity of fusion proteins can be followed by Tag-antibodies.
Very often, fusion proteins are directly injected into animals to generate antibodies.
Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000-1:10,000Western Blot : 1:1000-1:5000Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography conjugated with periodate-oxidated horseradish peroxidase
Form：Supplied as a lyophilized powder,, 0.1% Prolcin-300. Reconstitute with 100ul sterile PBS. Labeled with horseradish peroxidase (HRP). E/P ratio = 4.0.
Specificity：Recognizes c-Myc tag.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa410-419 near the C-Terminal of human myc protein, conjugated to KLH
