Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis.
Actin is one of the most conserved cellular protein.
At least 6 actin isoforms have been identified by protein sequence analyses.
Four actin isoforms represent the differentiation markers of muscle tissues.
There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g- smooth muscle and g-non-muscle).
Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60% homology).
Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human Beta-Actin. Species sequence homology: mouse, rat, human, chicken, frog.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Beta-Actin.
