84-1174-63　Actin, beta (Muscle) 100ul　A0759-08N

特徴

• Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis.
• Actin is one of the most conserved cellular protein.
• At least 6 actin isoforms have been identified by protein sequence analyses.
• Four actin isoforms represent the differentiation markers of muscle tissues.
• There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one bactin b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle).
• Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60 homology).
• Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000 (ECL)ELISA: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.