84-1174-62　ATP Binding Cassette subfamily G, Member 5, (ABCG5, Sterolin-1) 100ul　A0004-05C

特徴

• The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics.
• The first known members were Pglycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drugsensitive cells.
• In addition, increasing numbers of ABC proteins have recently been identified.
• The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules.
• ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.
• ABCG5 gene product a 651aa residue in human, and 652aa each in rat and mouse, plays an important role in the selective transport of cholesterol in and out of the enterocytes and in the selective sterol excretion by liver into bile.
• Strongly expressed in liver, intestine and colon.
• Defects in ABCG5 results increased intestinal absorption of sterols and decreased biliary excretion of dietary sterols into bile; the condition is referred as sitosterolemia.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (ECL)ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.