Hakai is an E-cadherin binding protein, which has been identified as an E3 ubiquitin-ligase.
Hakai contains SH2, RING, zinc-finger and proline-rich domains.
Hakai interacts with E-cadherin in a tyrosine phosphorylation-dependent manner, inducing ubiquitination of the E-cadherin complex.
Expression of Hakai in epithelial cells disrupts cell-cell contacts and enhances endocytosis of E-cadherin and cell motility.
By promoting the endocytosis and dynamic recycling or destruction of E-cadherin complexes, Hakai is able to control epithelial-mesenchymal transitions under physiological and pathological conditions.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:50 Immunofluorescence: 1:50Western Blot: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
