HE4 (also WDFC2; WAP Four-disulfide core domain protein 2) is a secreted, 25kD glycoprotein member of the Whey Acidic Protein family of molecules.
It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium.
It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase).
Although ovarian cuboidal epithelium does not express HE4, ovarian epithelial tumors do, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer.
Mature human HE4 is 94aa in length.
It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa31-73 and 74-123).
There are four potential splice variants.
One shows a deletion of aa27-74, while three others show aa substitutions: 28aa for aa75-124, 23aa for aa1-74, and 10aa for aa71-124.
Over aa31-124, human HE4 shares 63% aa identity with an extended (144 aa) mouse form of mature HE4.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5ug/mlELISA (direct): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium azide.
Specificity：Recognizes human WFDC2. Species sequence homology: mouse (63%).
