VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13kD member of the synaptobrevin family of proteins.
It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons.
VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I.
Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release.
Human VAMP-2 is 116aa in length.
It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa31-91) and a membrane-anchor domain (aa95-114).
Overaa1-94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human VAMP-2. Species Crossreactivity: mouse and rat. Species sequence homology: 100% canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Purified, E.coli-derived, recombinant human VAMP-2 (aa1-94; Accession # P63027).
