SPRY2 (sprouty homolog 2) is a 35kD member of the sprouty family of proteins.
It is widely expressed in embryonic and adult tissues, including endothelium.
SPRY2 is considered a negative regulator of Ras/ERK signaling.
For example, it appears to form a trimeric complex with PKC-delta and PKD1, blocking ERK phosphorylation.
It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability.
Human SPRY2 is 315 aa in length and contains one CBL-TKB binding site (aa53-59) that is phosphorylated at Tyr55, a Ser-rich region (aa125-131), a Cys-rich domain (aa178-301) and a PxxPxR motif that mediates ERK inhibitory activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% sodium azide.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium chloride.
Specificity：Recognizes human SPRY2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Purified, E. coli-derived recombinant human SPRY2 (rhSPRY2; aa1-175; Accession # O43597).
