SPRY1 (sprouty homolog 1) is a 34-38kD member of the sprouty family of proteins.
It is widely expressed, being found in multiple embryonic and adult tissues.
SPRY1 is considered a negative regulator of cellular signaling.
In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation.
It interacts with a number of molecules, including PLC-gama-1, LAT, CBL, caveolin-1 and SPRY2.
Human SPRY1 is 319 aa in length and contains one CBL-TKB binding site (aa51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa112-131), and a Cys-rich domain (aa181-306) that mediates intracellular translocation.
In addition, SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% NaN3.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.
Specificity：Recognizes human SPRY1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E.coli-derived recombinant human SPRY1 (aa1-178; Accession # O43609).
