Sirtuin 5 (SIRT5; SIR2-like protein 5) is a 28-35kD class III member of the sirtuin protein family.
It is found in the mitochondrial matrix and is involved in ammonia detoxification.
In particular, it deacetylates carbamoyl phosphate synthetase 1/CPS1, thus activating it and allowing for ammonia condensation with bicarbonate and ATP.
This is a crucial step in urea formation during prolonged fasting or the use of a high-protein diet.
Human SIRT5 is 310aa in length.
It possesses a sertuin-type deacetylase domain (aa41-309) that contains an NAD binding motif (Thr276:Glu277:Pro292).
There are three potential isoform variants that show aa substitutions at the C-terminus.
One contains a 14aa substitution for aa286-310, a second shows a 15aa substitution for aa287-310, and the third shows a 17aa substitution for aa207-310.
Full-length human and mouse SIRT5 share 86% aa identity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Specificity：Recognizes human SIRT5. Species sequence homology: mouse (86%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Purified, Sf 21-derived, recombinant human SIRT5 isoform 1 (rhSIRT5; aa33-310; Accession # Q9NXA8).
