RIBP (Rlk/Itk-binding protein; also SH2D2A, LAD/Lck-associated adapter protein, MEKK2-binding protein and TSAd) is a 45-47kD cytoplasmic adaptor protein.
It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR-initiated proliferation, plus IL-2 and IFN-gamma secretion.
This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site.
Mouse RIBP is 374aa in length and contains one SH2 domain (aa116-207), plus a Pro-rich region (aa267-308) that contains an SH3-binding motif (aa267-273).
Phosphorylation may occur on Ser237, Ser316 and Tyr325.
There is one alternative splice form termed LAD that shows a deletion of aa254-261.
Over aa299-374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml Detects a band at ~45kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% sodium azide.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.
Specificity：Recognizes mouse SH2D2A. Species Crossreactivity: rat.
