The semaphorins make up a family of inhibitory axon guidance molecules that include secreted, transmembrane, and GPI anchored extracellular molecules.
Via their interaction with Plexin/Neuropilin receptor complexes, the semaphorins are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets.
Semaphorin 3F belongs to the class 3 secreted group of molecules and shows ubiquitous expression in a range of neuronal and non-neuronal tissues including brain, mammary gland, liver, kidney, lung, spleen, muscle and heart.
The role of the semaphorins in non-neuronal tissues is poorly characterised.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Specificity：Recognizes human Semaphorin 3F. Species Crossreactivity: mouse.
