PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70kD/CAP70) is a 65-70kD member of the NHER (Na/H Exchange Regulator) family of proteins.
It is only expressed in epithelium, particularly that found in intestine, kidney and liver.
PDZK1 serves as a scaffold protein that connects cell membrane proteins to cytoplasmic regulators.
It also serves a critical role in basal-level bicarbonate secretion.
Mouse PDZK1 is 519aa in length and contains four PDZ domains (aa9-90; 128-215; 243-323; and 378-458) plus three phosphorylation sites at Ser489, Ser492 and Ser514.
Mouse PDZK1 shares 78% and 91% aa identity with human and rat PDZK1, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% sodium azide.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.
Specificity：Recognizes mouse PDZK1. Species Crossreactivity: rat.
