Neurocan (Neural proteoglycan; also CSPG3) is a secreted, 300-350kD proteoglycan member of the lectican family of molecules.
It is expressed by neurons and astrocytes, and has multiple binding partners, including heparin, FGF2, HB-CAM, glypican-1, syndecan-3, and tenascin-C plus -R.
Its expression is highest during development where it regulates neuronal migration and neurite outgrowth.
Mature mouse Neurocan is 1246aa in length.
It is highly modular, containing a V-type Ig-like domain (aa43-157), two link modules (aa159-356), two EGF-like domains (aa960-1034), a C-type lectin region (aa1036-1165), and a Sushi domain (aa1165-1225).
Cleavage occurs between Met643 and Leu644, generating a 621aa, 170-190kD N-terminal fragment, and a 625aa, 180kD C-terminal fragment.
Without proteoglycan adducts, SDS-page shows full-length Neurocan to be 245-260kD in size, with the N-terminal fragment 130kD, and the C-terminal fragment 150kD in size.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Specificity：Recognizes mouse Neurocan, Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Purified, CHO cell-derived, recombinant mouse Neurocan (aa23-637; Accession # NP_031815).
