Nogo-B (A No-Go for neurite outgrowth Isoform B; also reticulon-4 isoform 2 and RTN-xS) is a 49-51kD member of the reticulon protein family.
It is widely expressed, being reported everywhere but liver.
Nogo-B appears to be principally expressed in the ER, but does have a receptor (NgBR) on cell surfaces.
Intracellularly, Nogo-B is known to interact with Bcl-xl and Bcl-2, and may play in role in both apoptosis and angiogenesis.
Human Nogo-B is a two transmembrane, 373aa protein.
It contains an N-terminal cytoplasmic domain (aa1-199), two transmembrane segments (aa200-219 and 315-335), a luminal region (aa220-314) and a C-terminal cytoplasmic domain (aa336-373).
Nogo-B contains multiple phosphorylation sites and undergoes acetylation.
Caspase-7 cleaves Nogo-B between Asp14Ser15, generating a 45-47kD fragment.
Nogo-B is generated from a full-length 205kD Nogo-A by the deletion ofaa186-1004.
Overaa1-200, human Nogo-B shares 87% aa identity with mouse Nogo-B1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% NaN3.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human Nogo-B. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：CHO cell-derived, recombinant human Nogo-B (rhNogo-B; aa1-200; Accession #NP_722550).
