Nestin is a 240kD class VI intermediate filament protein that was first identified in neuroepithelial stem cells.
It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations.
In addition, Nestin has been found to be a marker for multi-lineage progenitor cells.
Over the region used for immunization, rat Nestin shares 76% aa sequence homology with the mouse protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Specificity：Recognizes rat Nestin. Species sequence homology: mouse (76%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Purified, E. coli-derived, recombinant rat Nestin (rrNestin; aa544-776).
