p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family.
NGF R cDNA encodes a 427aa residue precursor protein with a 28aa residue signal peptide, a 222aa residue extracellular domain, a 22aa residue transmembrane domain and a 155aa residue intracellular domain.
The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3 and NT-4 approximately equally with low affinity.
The cytoplasmic region contains a subtype 2 death domain.
NGF R expression has been shown to occur widely during development and in the adult.
Expression has been detected in both neuronal and non-neuronal cells.
NGF R was originally reported to function as a positive regulator of TrkA activity.
NGF R has also been shown to signal by itself.
Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis.
Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist.
Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using recombinant human NGF R/TNFRSF16 Fc ChimeraELISA: 0.1-0.4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 50ug BSA.Reconstitute with 250ul sterile PBS. Labeled with Biotin.
Specificity：Recognizes human Nerve Growth Factor Receptor, p75. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding Lys29-Asn250 at the extracellular domain of human NGF R/TNFRSF16, expressed in Sf21 cells.
