84-1174-23　MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45, OF45) 100ug　M2904-75

特徴

• MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56-58kD member of the SIBLING protein family.
• It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes.
• MEPE is found both intra- and extracellularly.
• Within the cell, it binds to CHK1.
• This protects CHK1 from degradation, and the cell from DNA damage.
• Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis.
• Undegraded, it promotes bone vascularization and blocks bone resorption.
• Following cleavage after Arg410 (in mouse), a 2kD ASARM fragment is generated that, when phosphorylated, blocks mineralization.
• Mature mouse MEPE is 417aa in length.
• It contains an RGD motif and ASARM domain (aa416-433).
• In addition to its 57kD form, 70kD, 42-45kD and 30-35kD forms of mouse MEPE have been reported.
• Whether they are cleavage forms or products of glycosylation is unclear.
• Over aa147-433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.
• Applications:Suitable for use in Direct ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml
• Direct ELISA: 0.5-1ug/ml.
• The detection limit is ~0.5ng/well
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.